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dna sequencer abi prism 3700 dna sequencer  (Thermo Fisher)


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    Thermo Fisher dna sequencer abi prism 3700 dna sequencer
    Dna Sequencer Abi Prism 3700 Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3700+sequencer/DNA/us12509524-2044-20-22
    Average 99 stars, based on 1 article reviews
    dna sequencer abi prism 3700 dna sequencer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A molecular assessment of species boundaries and relationships in the Australian brine shrimp Parartemia (Anostraca: Parartemiidae)
    Article Snippet: PCR reaction volumes were 25 μL containing 5 μL of GoTaq Reaction Buffer (Promega), 0.5 μL of dNTPs (10 mM per nucleotide), 0.25 μL of each of forward and reverse primers (10 μM), 2 μL of MgCl2 (25 mM), 0.35 μL of bovine serum albumin (10 μg μL–1), 0.125 μL of GoTaq G2 Hot Start Taq Polymerase, 1 μL of DNA and adjusted to the final volume using PCR grade water. .. PCR reactions for all three markers were (i) 95°C initial denaturation temperature for 5 min; (ii) 40 cycles of denaturation at 94°C for 1 min, annealing at 48°C for 1 min and extension at 72°C for 45 s; and (iii) a final extension at 72°C for 7 min. PCR products were purified using Exo-SAP purification (Dugan et al. 2002) and sequenced in the forward and reverse directions in an automatic ABI 3700 sequencer (Applied Biosystems) by Macrogen Inc. (South Korea). ..

    Article Title: Molecular Characterization of Mango (Mangifera indica L.) Cultivars Using SSR Markers
    Article Snippet: .. The diluted PCR products were separated by capillary electrophoresis on an ABI 3700 sequencer (Applied Biosystems). .. A set of 14 primer pairs was used in this study and synthesised by BIONEER Corporation, Korea.

    Article Title: Redescription and molecular phylogeny of Trochilia sigmoides Dujardin, 1841 (Ciliophora, Cyrtophoria) collected from South Korea.
    Article Snippet: During a survey of Korean marine ciliates, Trochilia sigmoides, the type species of the genus Trochilia, was collected and examined using in vivo observation and protargol impregnation.. Moreover, scanning electron microscopy and 18S rRNA gene sequencing have been applied for the first time to study this species.. Morphologically, T. sigmoides is characterized by the small body size, the oval body outline, and the spiral dorsal ridges.

    Purification:

    Article Title: A molecular assessment of species boundaries and relationships in the Australian brine shrimp Parartemia (Anostraca: Parartemiidae)
    Article Snippet: PCR reaction volumes were 25 μL containing 5 μL of GoTaq Reaction Buffer (Promega), 0.5 μL of dNTPs (10 mM per nucleotide), 0.25 μL of each of forward and reverse primers (10 μM), 2 μL of MgCl2 (25 mM), 0.35 μL of bovine serum albumin (10 μg μL–1), 0.125 μL of GoTaq G2 Hot Start Taq Polymerase, 1 μL of DNA and adjusted to the final volume using PCR grade water. .. PCR reactions for all three markers were (i) 95°C initial denaturation temperature for 5 min; (ii) 40 cycles of denaturation at 94°C for 1 min, annealing at 48°C for 1 min and extension at 72°C for 45 s; and (iii) a final extension at 72°C for 7 min. PCR products were purified using Exo-SAP purification (Dugan et al. 2002) and sequenced in the forward and reverse directions in an automatic ABI 3700 sequencer (Applied Biosystems) by Macrogen Inc. (South Korea). ..

    Sequencing:

    Article Title: Novel contribution to the taxonomy and molecular phylogeny of scuticociliates (Protozoa): Resolving the non-monophyly of the genus Paranophrys Thompson and Berger, 1965 and the establishment of the genus Anteparanophrys n. gen.
    Article Snippet: During a field survey of parasitic ciliates diversity in South Korea, a scuticociliate was found in a water sample collected during scuba diving.. At first glance, the species looks similar to members of the genus Paranophrys especially P. magna but they differ mainly in the body size and the number of somatic kineties.. Thus, integrative taxonomic approaches (i.e., silver impregnations, scanning electron microscopy, and molecular phylogeny) were applied and confirmed that the isolate is a new species.

    Article Title: Morphology and molecular phylogeny of Euplotes baugilensis n. sp. (Ciliophora, Spirotrichea), with an illustrated key to Euplotes species with reduced cirri.
    Article Snippet: Euplotes baugilensis n. sp. was discovered in a temporary puddle that formed after rainfall on a mountain footpath near Gangneung-Wonju National University in Gangneung, South Korea.. After isolation, a pure culture was established, and the new species was examined using live observation, silver-impregnation (protargol and ‘wet’ silver nitrate), scanning electron microscopy, and the analysis of the 18S rRNA gene sequence.. Morphologically, E. baugilensis n. sp. is characterized by small body size (on average 49 × 31 μm in vivo), 9 ordinary fronto-ventral cirri (cirrotype-9) with one reduced cirrus V/2 (composed of four non-ciliated basal bodies), 5 transverse cirri, 7 or 8 dorsolateral kineties, 6 dorsal prominent ridges, and a dargyrome (silverline system) of double type.

    Article Title: Redescription and molecular phylogeny of Trochilia sigmoides Dujardin, 1841 (Ciliophora, Cyrtophoria) collected from South Korea.
    Article Snippet: During a survey of Korean marine ciliates, Trochilia sigmoides, the type species of the genus Trochilia, was collected and examined using in vivo observation and protargol impregnation.. Moreover, scanning electron microscopy and 18S rRNA gene sequencing have been applied for the first time to study this species.. Morphologically, T. sigmoides is characterized by the small body size, the oval body outline, and the spiral dorsal ridges.

    Article Title: Met343Val mutation disrupts the shuttling of Trp380 leading to a low-activity conformer of activated protein C and causes thrombosis.
    Article Snippet: Genomic DNA was extracted from peripheral whole blood using the QIAamp DNA blood purification kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. Detection of genetic defects of PROC gene was carried out by sequencing using the ABI 3700 sequencer (Applied Biosystems, Foster City, CA). ..

    Electrophoresis:

    Article Title: Molecular Characterization of Mango (Mangifera indica L.) Cultivars Using SSR Markers
    Article Snippet: .. The diluted PCR products were separated by capillary electrophoresis on an ABI 3700 sequencer (Applied Biosystems). .. A set of 14 primer pairs was used in this study and synthesised by BIONEER Corporation, Korea.

    Clone Assay:

    Article Title: Redescription and molecular phylogeny of Trochilia sigmoides Dujardin, 1841 (Ciliophora, Cyrtophoria) collected from South Korea.
    Article Snippet: During a survey of Korean marine ciliates, Trochilia sigmoides, the type species of the genus Trochilia, was collected and examined using in vivo observation and protargol impregnation.. Moreover, scanning electron microscopy and 18S rRNA gene sequencing have been applied for the first time to study this species.. Morphologically, T. sigmoides is characterized by the small body size, the oval body outline, and the spiral dorsal ridges.

    DNA Sequencing:

    Article Title: Integrative studies shed light on the pseudocryptic genus Crassohymena n. gen. (Protozoa, Ciliophora).
    Article Snippet: The morphology, morphogenesis, molecular phylogeny, and the resting cyst morphology of the freshwater ciliate, Crassohymena primicirrata (Berger and Foissner, 1987) n. gen., n. comb., found in samples collected from South Korea, were investigated.. The Korean two populations observed are identical to each other and are highly similar to the type population showing only few and minor differences in the size and arrangement of cortical granules and the length of dorsal kinety 4.. The new genus Crassohymena n. gen. is established and characterized by a number of morphological and ontogenetic features: the thick undulating membranes comprising three or four rows of kinetosomes and in Cyrtohymena pattern; the anteriorly distinctly displaced transverse cirri; the posteriorly set-off cirrus V/3; the opisthe’s anlagen IV–VI originate from cirri IV/2, V/4, and V/3, respectively; the proter’s anlagen IV–VI originate from cirrus IV/3; and the enormous anlage I in each daughter cell.

    Article Title: Description of Conchophthirus sinanodontae n. sp. (Ciliophora: Scuticociliatia), a new endocommensal ciliate of the freshwater mussel Sinanodonta woodiana from Korea.
    Article Snippet: The morphology and molecular phylogeny of a new ciliate, Conchophthirus sinanodontae n. sp., which was discovered in the freshwater mussel Sinanodonta woodiana (Lea, 1834) from the Chilsancheon River, Buyeo-gun, South Korea, were investigated.. The new species was characterized and could be distinguished from congeners by a combination of characters including the ovate body outline, four to six oral polykinetids deeply embedded in the upper wall of the buccal cavity, six to ten vestibular kineties, 34–49 ventral and 36–53 dorsal somatic kineties.. The genetic differences among C. sinanodontae n. sp. and other congeners with available 18S rDNA sequences further support its distinctness.



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